生物学
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Activity-guided native-PAGE electroelution coupled with DIA proteomics uncovers the superoxide dismutase and antioxidant network in mature peanut seeds
作者Zihao Wei; Feng Huang; Yu Wang; Yuqiang Sun; Wangfeng Li; Wanli Guo
作者单位Molecular Biological Engineering Lab, Department of Biotechnology, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China; Laboratory of Molecular Precision Diagnosis, Chengdu Base Cipher Biotechnology Co., Ltd., No. 618, Fenghuang Road, Shuangliu District, Chengdu, Sichuan, China
Stable CHO production and functional validation of a recombinant canine NGF-Fc fusion protein
作者Zhimin Li; Yang Luan; Han Song; Hualin Xu; Yuyao Wu; Zhenwei Bi; Yeping Tan
作者单位Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biologicals Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio - Products, Nanjing, 210014, China; Xizang Agriculture and Animal Husbandry College, Linzhi, 860000, China; College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, 163000, China; College of Veterinary Medicine, Northwest Agriculture and Forestry University, Xianyang, 712100, China
Engineering 3′-UTR hairpin structures to modulate mRNA stability and recombinant protein production in Escherichia coli
作者Zukhra Khasanshina; Marina Yarovikova; Eugenia Buslaeva; Anton Filipenko; Roman Dray
作者单位Pharm-Holding (RnD GEROPHARM), P. Strelna 34-A Svyazi Street, St. Petersburg, 198515, Russia; ITMO University, St. Petersburg, 197101, Russia
Recombinant production, biochemical characterization, and in vitro evaluation of an EWS-FLI1-derived peptide for Ewing sarcoma
作者Yogalakshmi Sabapathy; Manasi Sivakami Kumar; Aravindh Sundaram Palani; Gopal Gopisetty
作者单位Cancer Institute (WIA), Chennai, India; Vellore Institute of Technology, India
Evaluation of induction conditions for multi-antigen chimeric TgAGS/BsT protein from Toxoplasma gondii in Escherichia coli BL 21 Star during batch cultivation
作者Stephanie Caroline Bivar Matias; José Daladiê Barreto da Costa Filho; Andrew Douglas Moura; Daniella Regina Arantes Martins; Francisco Canindé de Sousa Júnior; Everaldo Silvino dos Santos
作者单位Department of Chemical Engineering, Technology Center, Federal University of Rio Grande do Norte, Avenue Senador Salgado Filho, 3000, Lagoa Nova, Natal, 59072-970, Brazil; Department of Biochemistry, Biosciences Center, Federal University of Rio Grande do Norte, Avenue Senador Salgado Fd00, Lagoa Nova, Natal, 59072-970, Brazil; Department of Pharmacy, Federal University of Rio Grande do Norte, Street General Gustavo Cordeiro de Farias, Petrópolis, Natal, 59012-570, Brazil
Expression and purification of recombinant Vibrio cholerae σ38, σ54 and RNAP: Insights into homotypic oligomerization and holoenzyme assembly barriers
作者Abdul Basit Khan; Shivam Sahu; Pragati Agnihotri; Anil Kumar Shakya; Surendra Saw; J. Venkatesh Pratap
作者单位Biochemistry and Structural Biology Division, CSIR- Central Drug Research Institute, Sector-10, Jankipuram Extension, Lucknow, UP, 226031, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, UP, 201002, India
Expression, purification, crystallization and structure solution of glyceraldehyde-3-phosphate dehydrogenase from the babesiosis infective agent Babesia bovis
作者Bruna Ukrainski; Emelly B. Galvão; Marcio Silva; Jorge Iulek
作者单位Chemistry Departament, State University of Ponta Grossa, Paraná, Brazil; Department of Pharmaceutical Sciences, State University of Ponta Grossa, Paraná, Brazil; Academic Teaching Department, Federal Technological University of Paraná, Paraná, Brazil
Optimization of a DEAE ion-exchange workflow for a porcine brain-derived vimentin-containing preparation
作者Xiang Gao; Yukun Ma; Xing Chen; Yuanyuan Yang; Yun Wang; Nian Wang
作者单位Department of Pharmacy, Medical Support Center, The 902nd Hospital of the PLA Joint Logistics Support Force, Bengbu, Anhui Province, China; Unit for Drug and Instrument Supervision and Inspection of Wuxi Joint Logistic Support Center, Nanjing, Jiangsu, 210000, China
Human TBC1 domain-containing kinase is a class I multidomain pseudokinase
作者Shiwangi Maurya; Lilly E. Cheek; Anthony T. Iavarone; Wen Zhu
作者单位Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, 32306, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, CA, 94720, USA
Simultaneous solubility-enhancing domain removal and target purification using the Npu split intein
作者Sophia Mayone; Shanthi K. Napier; Roman Adomanis; Emily Baines; Deepak T. Patel; Jordan C. Loeffler; Reva Sharma; Milton S. Gonzalez-Serrano; Blaise R. Kimmel; Cameron Semper; David W. Wood
作者单位The Ohio State University William G Lowrie Department of Chemical and Biomolecular Engineering, 151 West Woodruff Ave., Columbus, OH, 43210, USA; Department of Microbiology, Immunology and Infectious Disease, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N N14, Canada
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